| Test Number | 087 |
| Method | Indirect Immunofluorescence (IFA) |
| Reference Range |
Qualitative
|
| CPT Code | 83516 |
| Schedule Turnaround Time | Assay performed once every two weeks. Report availability is two weeks from the time of specimen receipt. |
| Specimen Requirements | Specimen need not be refrigerated or frozen. Collect 4-10 ml of blood in a red top or serum separator tube. If possible, separate serum from clot and place into orange tube provided with IMMCO collection kits. Do not puncture top of orange tube. If separation facilities are not available, the blood can be sent in the tube used for collection.
Requested Sample Volume: 4 mL
Minimum Sample Volume: 0.5mL
|
| Sample Stability | Sample is stable at ambient temperature during shipment. If sample is stored prior to shipment, it is stable refrigerated (2-8˚C) up to five days and frozen (-20˚C or lower) up to one year. |
| Clinical Relevance | Anti-PM/Scl antibodies are present in sera from patients with polymyositis (PM), systemic sclerosis (SSc), and PM/SSc overlap syndromes. The prevalence of antibodies against the 75- and 100-kDa PM/Scl proteins and their clinical association in SSc patients revealed a diagnostic sensitivity of 12.5% and a specificity of 96.9% for SSc. Among anti-PM/Scl-positive, 7.1% were positive for -PM/Scl-100 antibodies. |
| Antibodies |
|
| Autoimmune Diseases |
- Dermato/Polymyositis
- Dermatopathology
|